Multi-Stage Analytical Product Testing
How Peptide Verge UK verifies molecular identity, chromatographic purity, gravimetric fill accuracy, and contaminant thresholds across analytical peptide lots.
1. Reversed-Phase HPLC-UV Purity (≥99%)
C18 reversed-phase gradient elution with UV photodiode detection (214 nm / 220 nm) quantifies peak area integration against synthesis deletion sequences and trifluoroacetate counter-ions.
2. Electrospray Ionization Mass Spectrometry (LC-MS)
Multi-charged ion deconvolution ([M+H]+, [M+2H]2+, [M+3H]3+) confirms exact monoisotopic and average molecular mass against the theoretical amino acid sequence.
3. USP <85> Bacterial Endotoxin (LAL) & PCR
Selected cellular-assay lots undergo duplicate Limulus Amebocyte Lysate (LAL) testing (≤0.05 EU/mL sensitivity) and PCR microbial DNA screening so primary cell cultures remain free of lipopolysaccharide interference.
4. ICP-MS Elemental Impurity Screening
Inductively Coupled Plasma Mass Spectrometry screens Arsenic (As), Cadmium (Cd), Lead (Pb), and Mercury (Hg) below ICH Q3D / USP <232> specification thresholds.